Digestion of micellar casein in duodenum cannulated pigs. Correlation between in vitro simulated gastric digestion and in vivo data

Correlation and validation of the results simulated gastrointestinal digestion food compounds towards in vivo data is essential. The objective this work was to monitor milk micellar casein porcine upper intestinal tract match outcome with gastric vitro following Infogest harmonized protocol. In pig duodenum, small amounts intact caseins were present all samples, while observed up 60 min digestion. peptide profile generated after showed clear similarities specific overrepresented regions rich proline other hydrophobic residues. statistical comparison peptidome resulted satisfactory correlation coefficients, 0.8. Therefore, protocol used a robust simple model that provides similar than found duodenum.